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KMID : 0380619930250020089
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Korean Journal of Food Science and Technology
1993 Volume.25 No. 2 p.89 ~ p.93
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Production of β - xylosidase from Bifidobacterium sp . Int - 57
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Lee Ke-Ho
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Abstract
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In order to study the effect of the intestinal bacteria on the physiology of the human large intestinal tract, we isolated the intestinal bacteria of Koreans and tested the enzymatic patterns. Isolated Bifidobacterium sp. Int-57 showed the higher activity of β-xylosidase than other intestinal microorganisms. The effect of the carbon sources, nitrogen sources, inorganic salts, initial pH and initial temperature on the production of β-xylosidase of Bifidobacterium sp. Int-57 was investigated. The most suitable carbon source, nitrogen source and inorganic salt for the production of β-xylosidase were 1.1% xylose, 0.4% yeast extract and 0.0003% CoCl₂ respectively at initial pH 7.0 and temperature 40℃.
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